SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSNA3
Systematic nameYJL151C
DescriptionProtein SNA3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K125        
2K125        
3         
4K19;K125MULTI HECTNARSP5 DOA4 
5   HECTNARSP5   
6K125MULTI(K63-linked) HECTNARSP5 DOA4Vacuolar Degradation
7         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS
Reference"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[3]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[4]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Direct binding to Rsp5 mediates ubiquitin-independent sorting of Sna3 via the multivesicular body pathway."
McNatt MW, McKittrick I, West M, Odorizzi G.
Mol Biol Cell. 18(2):697-706 (2007) [17182850]
[5]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]
[6]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Targeting of Sna3p to the Endosomal Pathway Depends on Its Interaction with Rsp5p and Multivesicular Body Sorting on Its Ubiquitylation."
Stawiecka-Mirota M, Pokrzywa W, Morvan J, Zoladek T, Haguenauer-Tsapis R, Urban-Grimal D, Morsomme P.
Traffic. 8(9):1280-96 (2007) [17645729]
[7]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP14359
SGD YJL151C
Gene Ontology (GO)
Cellular componentGO:0000328lumen of vacuole with cell cycle-correlated...IDA:SGD.
Cellular componentGO:0016020membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
86Phosphothreonine.

Sequence Information
Sequence length133
Molecular weight15199

	---------+----------+----------+----------+----------+
MDRDHINDHD HRMSYSINKD DLLLMVLAVF IPPVAVWKRK GMFNRDTLLN   50
LLLFLLLFFP AIIHACYVVY ETSSERSYDL SRRHATAPAV DRDLEAHPAE   100
ESQAQPPAYD EDDEAGADVP LMDNKQQLSS GRT                     133

	* Ubiquitinated lysine is denoted as red color.