SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSMF2
Systematic nameYHR050W
DescriptionManganese transporter SMF2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38778
SGD YHR050W
Gene Ontology (GO)
Cellular componentGO:0016023cytoplasmic membrane-bound vesicleIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0005384manganese ion transporter activityIGI:SGD.
Biological processGO:0006828manganese ion transportIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length549
Molecular weight59768

	---------+----------+----------+----------+----------+
MTSQEYEPIQ WSDESQTNND SVNDAYADVN TTHESRRRTT LQPNSTSQSM   50
IGTLRKYARF IGPGLMVSVS YMDPGNYSTA VAAGSAHRYK LLFSVLVSNF   100
MAAFWQYLCA RLGAVTGLDL AQNCKKHLPF GLNITLYILA EMAIIATDLA   150
EVVGTAISLN ILFHIPLALG VILTVVDVLI VLLAYKPNGS MKGIRIFEAF   200
VSLLVVLTVV CFTVELFYAK LGPAKEIFSG FLPSKAVFEG DGLYLSLAIL   250
GATVMPHSLY LGSGVVQPRL REYDIKNGHY LPDANDMDNN HDNYRPSYEA   300
ISETLHFTIT ELLISLFTVA LFVNCAILIV SGATLYGSTQ NAEEADLFSI   350
YNLLCSTLSK GAGTVFVLAL LFSGQSAGIV CTLSGQMVSE GFLNWTVSPA   400
LRRSATRAVA ITPCLILVLV AGRSGLSGAL NASQVVLSLL LPFVSAPLLY   450
FTSSKKIMRV QLNRTKELSR TTDKKPVADR TEDDETIELE EMGIGSSSQE   500
RSLVSPAPEY KDMSNGMIVT VLAIIVWLII SGLNFYMLLG FTTGKEVHL    549