SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSMC3
Systematic nameYJL074C
DescriptionStructural maintenance of chromosomes protein 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP47037
SGD YJL074C
Gene Ontology (GO)
Cellular componentGO:0000798nuclear cohesin complexTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007064mitotic sister chromatid cohesionIMP:SGD.
Biological processGO:0030437sporulation (sensu Fungi)IMP:SGD.
Biological processGO:0007130synaptonemal complex assemblyIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1230
Molecular weight141336

	---------+----------+----------+----------+----------+
MYIKRVIIKG FKTYRNETII DNFSPHQNVI IGSNGSGKSN FFAAIRFVLS   50
DDYSNLKREE RQGLIHQGSG GSVMSASVEI VFHDPDHSMI LPSGVLSRGD   100
DEVTIRRTVG LKKDDYQLND RNVTKGDIVR MLETAGFSMN NPYNIVPQGK   150
IVALTNAKDK ERLQLLEDVV GAKSFEVKLK ASLKKMEETE QKKIQINKEM   200
GELNSKLSEM EQERKELEKY NELERNRKIY QFTLYDRELN EVINQMERLD   250
GDYNNTVYSS EQYIQELDKR EDMIDQVSKK LSSIEASLKI KNATDLQQAK   300
LRESEISQKL TNVNVKIKDV QQQIESNEEQ RNLDSATLKE IKSIIEQRKQ   350
KLSKILPRYQ ELTKEEAMYK LQLASLQQKQ RDLILKKGEY ARFKSKDERD   400
TWIHSEIEEL KSSIQNLNEL ESQLQMDRTS LRKQYSAIDE EIEELIDSIN   450
GPDTKGQLED FDSELIHLKQ KLSESLDTRK ELWRKEQKLQ TVLETLLSDV   500
NQNQRNVNET MSRSLANGII NVKEITEKLK ISPESVFGTL GELIKVNDKY   550
KTCAEVIGGN SLFHIVVDTE ETATLIMNEL YRMKGGRVTF IPLNRLSLDS   600
DVKFPSNTTT QIQFTPLIKK IKYEPRFEKA VKHVFGKTIV VKDLGQGLKL   650
AKKHKLNAIT LDGDRADKRG VLTGGYLDQH KRTRLESLKN LNESRSQHKK   700
ILEELDFVRN ELNDIDTKID QVNGNIRKVS NDRESVLTNI EVYRTSLNTK   750
KNEKLILEES LNAIILKLEK LNTNRTFAQE KLNTFENDLL QEFDSELSKE   800
EKERLESLTK EISAAHNKLN ITSDALEGIT TTIDSLNAEL ESKLIPQEND   850
LESKMSEVGD AFIFGLQDEL KELQLEKESV EKQHENAVLE LGTVQREIES   900
LIAEETNNKK LLEKANNQQR LLLKKLDNFQ KSVEKTMIKK TTLVTRREEL   950
QQRIREIGLL PEDALVNDFS DITSDQLLQR LNDMNTEISG LKNVNKRAFE   1000
NFKKFNERRK DLAERASELD ESKDSIQDLI VKLKQQKVNA VDSTFQKVSE   1050
NFEAVFERLV PRGTAKLIIH RKNDNANDHD ESIDVDMDAE SNESQNGKDS   1100
EIMYTGVSIS VSFNSKQNEQ LHVEQLSGGQ KTVCAIALIL AIQMVDPASF   1150
YLFDEIDAAL DKQYRTAVAT LLKELSKNAQ FICTTFRTDM LQVADKFFRV   1200
KYENKISTVI EVNREEAIGF IRGSNKFAEV                          1230