SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSLT2
Systematic nameYHR030C
AliasMPK1
DescriptionMitogen-activated protein kinase SLT2/MPK1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ00772
SGD YHR030C
Gene Ontology (GO)
Cellular componentGO:0005934cellular bud tipIDA:SGD.
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007047cell wall organization and biogenesisTAS:SGD.
Biological processGO:0008361regulation of cell sizeIMP:SGD.
Biological processGO:0001101response to acidIMP:SGD.
Biological processGO:0030968unfolded protein responseIDA:SGD.
EC number 2.7.11.24

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
190Phosphothreonine.
192Phosphotyrosine.

Sequence Information
Sequence length484
Molecular weight55636

	---------+----------+----------+----------+----------+
MADKIERHTF KVFNQDFSVD KRFQLIKEIG HGAYGIVCSA RFAEAAEDTT   50
VAIKKVTNVF SKTLLCKRSL RELKLLRHFR GHKNITCLYD MDIVFYPDGS   100
INGLYLYEEL MECDMHQIIK SGQPLTDAHY QSFTYQILCG LKYIHSADVL   150
HRDLKPGNLL VNADCQLKIC DFGLARGYSE NPVENSQFLT EYVATRWYRA   200
PEIMLSYQGY TKAIDVWSAG CILAEFLGGK PIFKGKDYVN QLNQILQVLG   250
TPPDETLRRI GSKNVQDYIH QLGFIPKVPF VNLYPNANSQ ALDLLEQMLA   300
FDPQKRITVD EALEHPYLSI WHDPADEPVC SEKFEFSFES VNDMEDLKQM   350
VIQEVQDFRL FVRQPLLEEQ RQLQLQQQQQ QQQQQQQQQQ QPSDVDNGNA   400
AASEENYPKQ MATSNSVAPQ QESFGIHSQN LPRHDADFPP RPQESMMEMR   450
PATGNTADIP PQNDNGTLLD LEKELEFGLD RKYF                    484