SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSKG1
Systematic nameYKR100C
DescriptionSuppressor of lethality of KEX2 GAS1 double null mutant protein 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP36169
SGD YKR100C
Gene Ontology (GO)
Cellular componentGO:0005934cellular bud tipIDA:SGD.
Cellular componentGO:0000131incipient cellular bud siteIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007047cell wall organization and biogenesisIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
256Phosphoserine.
257Phosphoserine.
260Phosphoserine.

Sequence Information
Sequence length355
Molecular weight39830

	---------+----------+----------+----------+----------+
MTASTSVAVG CAVGIPVGVG IIIAVCFWFN LQKRYKREEQ DDRELERAIY   50
DESGFVSFDN FGPLRDSKDE AALASSELKN PDHTSGSSEG SAHPEEKDGK   100
SRDQEKPLGK KNSKYYVPAY RRKINLLQVR NNNYGNNARQ KSVVDLPSIN   150
NSSNVSLSSS QRHITKRQIS VYDQMVPVIS DEGPNFFADP SSDTNTSNDQ   200
NKASMIELKH NTRQSSNENL IRNLQNQDFG SYYPRRASSS FLNGNISNAS   250
FHTRNSSITS VNKRDALEDV FATPKSAAQS QLPNTFDKDN EGMDADHSVK   300
DSRSAITDKD KDLYKLQNNY DVGNIGEIAE EDQYENEFTN YSQSKREFIE   350
SLRPK                                                    355