SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSIS1
Systematic nameYNL007C
DescriptionProtein SIS1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP25294
SGD YNL007C
Gene Ontology (GO)
Cellular componentGO:0005843cytosolic small ribosomal subunit (sensu Eu...IDA:SGD.
Molecular functionGO:0030188chaperone regulator activityIDA:SGD.
Molecular functionGO:0051082unfolded protein bindingTAS:SGD.
Biological processGO:0006457protein foldingIDA:SGD.
Biological processGO:0006413translational initiationIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
173Phosphoserine.

Sequence Information
Sequence length352
Molecular weight37590

	---------+----------+----------+----------+----------+
MVKETKLYDL LGVSPSANEQ ELKKGYRKAA LKYHPDKPTG DTEKFKEISE   50
AFEILNDPQK REIYDQYGLE AARSGGPSFG PGGPGGAGGA GGFPGGAGGF   100
SGGHAFSNED AFNIFSQFFG GSSPFGGADD SGFSFSSYPS GGGAGMGGMP   150
GGMGGMHGGM GGMPGGFRSA SSSPTYPEEE TVQVNLPVSL EDLFVGKKKS   200
FKIGRKGPHG ASEKTQIDIQ LKPGWKAGTK ITYKNQGDYN PQTGRRKTLQ   250
FVIQEKSHPN FKRDGDDLIY TLPLSFKESL LGFSKTIQTI DGRTLPLSRV   300
QPVQPSQTST YPGQGMPTPK NPSQRGNLIV KYKVDYPISL NDAQKRAIDE   350
NF                                                       352