SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSIR4
Systematic nameYDR227W
AliasASD1, STE9, UTH2
DescriptionRegulatory protein SIR4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2   RINGSPRFSAN1   
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Sir Antagonist 1 (San1) is a ubiquitin ligase."
Dasgupta A, Ramsey KL, Smith JS, Auble DT.
J Biol Chem. 279(26):26830-8 (2004) [15078868]

Cross References
Swiss-Prot accessionP11978
SGD YDR227W
Gene Ontology (GO)
Cellular componentGO:0005677chromatin silencing complexIDA:SGD.
Cellular componentGO:0000783nuclear telomere cap complexIDA:SGD.
Cellular componentGO:0005724nuclear telomeric heterochromatinIDA:SGD.
Molecular functionGO:0042393histone bindingTAS:SGD.
Molecular functionGO:0030527structural constituent of chromatinTAS:SGD.
Biological processGO:0006342chromatin silencingTAS:SGD.
Biological processGO:0006303double-strand break repair via nonhomologou...IDA:SGD.
Biological processGO:0001308loss of chromatin silencing during replicat...IDA:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
62Phosphothreonine.
1134Phosphoserine.

Sequence Information
Sequence length1358
Molecular weight152062

	---------+----------+----------+----------+----------+
MPNDNKTPNR SSTPKFTKKP VTPNDKIPER EEKSNEVKTP KIPLFTFAKS   50
KNYSRPSTAI HTSPHQPSDV KPTSHKQLQQ PKSSPLKKNN YNSFPHSNLE   100
KISNSKLLSL LRSKTSAGRI ESNNPSHDAS RSLASFEQTA FSRHAQQQTS   150
TFNSKPVRTI VPISTSQTNN SFLSGVKSLL SEEKIRDYSK EILGINLANE   200
QPVLEKPLKK GSADIGASVI SLTKDKSIRK DTVEEKKEEK LNIGKNFAHS   250
DSLSVPKVSA GDSGISPEES KARSPGIAKP NAIQTEVYGI NEESTNERLE   300
INQEKPVKLD ENSANSTVAS ALDTNGTSAT TETLTSKKIV PSPKKVAIDQ   350
DKITLHDEKT LAPSKHQPIT SEQKMKEDAD LKRMEILKSP HLSKSPADRP   400
QGRRNSRNFS TRDEETTKLA FLVEYEGQEN NYNSTSRSTE KKNDMNTSAK   450
NKNGENKKIG KRPPEIMSTE AHVNKVTEET TKQIQSVRID GRKVLQKVQG   500
ESHIDSRNNT LNVTPSKRPQ LGEIPNPMKK HKPNEGRTPN ISNGTINIQK   550
KLEPKEIVRD ILHTKESSNE AKKTIQNPLN KSQNTALPST HKVTQKKDIK   600
IGTNDLFQVE SAPKISSEID RENVKSKDEP VSKAVESKSL LNLFSNVLKA   650
PFIKSESKPF SSDALSKEKA NFLETIASTE KPENKTDKVS LSQPVSASKH   700
EYSDNFPVSL SQPSKKSFAN HTEDEQIEKK KICRGRMNTI ITHPGKMELV   750
YVSDSDDSSS DNDSLTDLES LSSGESNEIK VTNDLDTSAE KDQIQAGKWF   800
DPVLDWRKSD RELTKNILWR IADKTTYDKE TITDLIEQGI PKHSYLSGNP   850
LTSVTNDICS VENYETSSAF FYQQVHKKDR LQYLPLYAVS TFENTNNTEK   900
NDVTNKNINI GKHSQEQNSS SAKPSQIPTV SSPLGFEETK LSTTPTKSNR   950
RVSHSDTNSS KPKNTKENLS KSSWRQEWLA NLKLISVSLV DEFPSELSDS   1000
DRQIINEKMQ LLKDIFANNL KSAISNNFRE SDIIILKGEI EDYPMSSEIK   1050
IYYNELQNKP DAKKARFWSF MKTQRFVSNM GFDIQKSCEP VSISTSVKPH   1100
VVEPEHMADA KIMPKDILQI TKKPLMVKNV KPSSPPDVKS LVQLSTMETK   1150
TLPEKKQFDS IFNSNKAKII PGNGKHASEN ISLSFSRPAS YGYFSVGKRV   1200
PIVEDRRVKQ LDDITDSNTT EILTSVDVLG THSQTGTQQS NMYTSTQKTE   1250
LEIDNKDSVT ECSKDMKEDG LSFVDIVLSK AASALDEKEK QLAVANEIIR   1300
SLSDEVMRNE IRITSLQGDL TFTKKCLENA RSQISEKDAK INKLMEKDFQ   1350
VNKEIKPY                                                 1358