SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSIM1
Systematic nameYIL123W
AliasPBP3
DescriptionProtein SIM1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP40472
SGD YIL123W
Gene Ontology (GO)
Cellular componentGO:0009277chitin- and beta-glucan-containing cell wallIDA:SGD.
Biological processGO:0000226microtubule cytoskeleton organization and b...IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length475
Molecular weight48071

	---------+----------+----------+----------+----------+
MKFSTAVTTL ISSGAIVSAL PHVDVHQEDA HQHKRAVAYK YVYETVVVDS   50
DGHTVTPAAS EVATAATSAI ITTSVLAPTS SAAAGIAASI AVSSAALAKN   100
EKISDAAASA TASTSQGASS SSSSSSATST LESSSVSSSS EEAAPTSTVV   150
STSSATQSSA SSATKSSTSS TSPSTSTSTS TSSTSSSSSS SSSSSSSSSG   200
SGSIYGDLAD FSGPSEKFQD GTIPCDKFPS GQGVISIDWI GEGGWSGVEN   250
TDTSTGGSCK EGSYCSYSCQ PGMSKTQWPS DQPSDGRSVG GLLCKNGYLY   300
RSNTDADYLC EWGVEAAYVV SKLSKGVAIC RTDYPGTENM VIPTYVEGGS   350
SLPLTVVDQD TYFTWEGKKT SAQYYVNNAG VSVEDGCIWG TSGSGIGNWA   400
PLNFGAGSTG GVTYLSLIPN PNNSDALNYN VKIVAADDSS NVIGECVYEN   450
GEFSGGADGC TVSVTSGKAH FVLYN                              475