SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSIK1
Systematic nameYLR197W
AliasNOP56
DescriptionRibosome biosynthesis protein SIK1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2 MULTI    RPN10 Proteasomal Degradation
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ12460
SGD YLR197W
Gene Ontology (GO)
Cellular componentGO:0031428box C/D snoRNP complexIPI:SGD.
Biological processGO:000636535S primary transcript processingIMP:SGD.
Biological processGO:0030490processing of 20S pre-rRNAIPI:SGD.
Biological processGO:0000154rRNA modificationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
321Phosphoserine.

Sequence Information
Sequence length504
Molecular weight56864

	---------+----------+----------+----------+----------+
MAPIEYLLFE EPTGYAVFKV KLQQDDIGSR LKEVQEQIND FGAFTKLIEL   50
VSFAPFKGAA EALENANDIS EGLVSESLKA ILDLNLPKAS SKKKNITLAI   100
SDKNLGPSIK EEFPYVDCIS NELAQDLIRG VRLHGEKLFK GLQSGDLERA   150
QLGLGHAYSR AKVKFSVQKN DNHIIQAIAL LDQLDKDINT FAMRVKEWYG   200
WHFPELAKLV PDNYTFAKLV LFIKDKASLN DDSLHDLAAL LNEDSGIAQR   250
VIDNARISMG QDISETDMEN VCVFAQRVAS LADYRRQLYD YLCEKMHTVA   300
PNLSELIGEV IGARLISHAG SLTNLSKQAA STVQILGAEK ALFRALKTKG   350
NTPKYGLIYH SGFISKASAK NKGRISRYLA NKCSMASRID NYSEEPSNVF   400
GSVLKKQVEQ RLEFYNTGKP TLKNELAIQE AMELYNKDKP AAEVEETKEK   450
ESSKKRKLED DDEEKKEKKE KKSKKEKKEK KEKKDKKEKK DKKEKKDKKK   500
KSKD                                                     504