SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSIC1
Systematic nameYLR079W
AliasSDB25
DescriptionProtein SIC1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTICDC34RINGSCFCDC4   
2  CDC34RINGSCFCDC4   
3       RPN11Proteasomal Degradation
4K32;K36;K50;K53;K84;K88MULTICDC34RINGSCFCDC4  Proteasomal Degradation
5  CDC34, UBC4RINGSCFCDC4   
6 MULTI    RPN10 Proteasomal Degradation
7 MULTI(K48-linked)CDC34RINGSCFCDC4   
* Blank: not determined, NA: not applicable.

References
[1]Evidence 
Method 
Reference"SIC1 is ubiquitinated in vitro by a pathway that requires CDC4, CDC34, and cyclin/CDK activities."
Verma R, Feldman RM, Deshaies RJ.
Mol Biol Cell. 8(8):1427-37 (1997) [9285816]
[2]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"F-box proteins are receptors that recruit phosphorylated substrates to the SCF ubiquitin-ligase complex."
Skowyra D, Craig KL, Tyers M, Elledge SJ, Harper JW.
Cell. 91(2):209-19 (1997) [9346238]
[3]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Role of Rpn11 metalloprotease in deubiquitination and degradation by the 26S proteasome."
Verma R, Aravind L, Oania R, McDonald WH, Yates JR 3rd, Koonin EV, Deshaies RJ.
Science. 298(5593):611-5 (2002) [12183636]
[4]EvidenceHigh MW Product Confirmation
MethodWestern blot
Reference"Context of multiubiquitin chain attachment influences the rate of Sic1 degradation."
Petroski MD, Deshaies RJ.
Mol Cell. 11(6):1435-44 (2003) [12820958]
[5]EvidenceHigh MW Product Confirmation
MethodWestern blot (in vitro)
Reference"Functional interaction of 13 yeast SCF complexes with a set of yeast E2 enzymes in vitro."
Kus BM, Caldon CE, Andorn-Broza R, Edwards AM.
Proteins. 54(3):455-67 (2004) [14747994]
[6]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[7]Evidence 
Method 
Reference"From loops to chains: unraveling the mysteries of polyubiquitin chain specificity and processivity."
Sowa ME, Harper JW.
ACS Chem Biol. 1(1):20-4 (2006) [17163635]

Cross References
Swiss-Prot accessionP38634
SGD YLR079W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004861cyclin-dependent protein kinase inhibitor a...IDA:SGD.
Biological processGO:0000082G1/S transition of mitotic cell cycleTAS:SGD.
Biological processGO:0000079regulation of cyclin-dependent protein kina...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
5Phosphothreonine, by PHO85.
33Phosphothreonine.
76Phosphoserine.
173Phosphothreonine.
198Phosphoserine.
201Phosphoserine.
268Lysine derivative.
272Lysine derivative.
274Lysine derivative.

Sequence Information
Sequence length284
Molecular weight32223

	---------+----------+----------+----------+----------+
MTPSTPPRSR GTRYLAQPSG NTSSSALMQG QKTPQKPSQN LVPVTPSTTK   50
SFKNAPLLAP PNSNMGMTSP FNGLTSPQRS PFPKSSVKRT LFQFESHDNG   100
TVREEQEPLG RVNRILFPTQ QNVDIDAAEE EEEGEVLLPP SRPTSARQLH   150
LSLERDEFDQ THRKKIIKDV PGTPSDKVIT FELAKNWNNN SPKNDARSQE   200
SEDEEDIIIN PVRVGKNPFA SDELVTQEIR NERKRAMLRE NPDIEDVITY   250
VNKKGEVVEK RRLTDEEKRR FKPKALFQSR DQEH                    284

	* Ubiquitinated lysine is denoted as red color.