SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSHQ1
Systematic nameYIL104C
DescriptionProtein SHQ1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP40486
SGD YIL104C
Gene Ontology (GO)
Cellular componentGO:0005654nucleoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0016074snoRNA metabolic processIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length507
Molecular weight59151

	---------+----------+----------+----------+----------+
MITPRFSITQ DEEFIFLKIF ISNIRFSAVG LEIIIQENMI IFHLSPYYLR   50
LRFPHELIDD ERSTAQYDSK DECINVKVAK LNKNEYFEDL DLPTKLLARQ   100
GDLAGADALT ENTDAKKTQK PLIQEVETDG VSNNIKDDVK TIGQMGEGFN   150
WEIEQKMDSS TNNGILKTKY GFDNLYDTVI SVSTSNGNDI NELDDPEHTD   200
ANDRVIERLR KENLKFDPEY YVSEYMTHKY GNEEDLEING IKELLKFTPS   250
IVKQYLQWYK DSTNPNLVMP IEFTDEEQKQ MQDNLPKKSY LVEDIKPLYV   300
TILSVLFSYV FEQIENEGTH TTESAWTMGK LCPQISFLDQ QLKQVNELQD   350
GMKEISKVNK DSSLIKIAII TGIRRALSYP LHRNYDLAMK AWTFVYYILR   400
GGKRLVIRAL LDIHETFRFH DVYYVYDKVL LDDLTAWFIS QGSENVIRSL   450
ALEMRKEQES LSKQDIEFEC IASFNEQTGE PEWETLNIRE MEILAESEYR   500
EQQQNPQ                                                  507