SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSHM2
Systematic nameYLR058C
AliasSHMT2
DescriptionSerine hydroxymethyltransferase, cytosolic

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP37291
SGD YLR058C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmTAS:SGD.
Molecular functionGO:0004372glycine hydroxymethyltransferase activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006730one-carbon compound metabolic processTAS:SGD.
EC number 2.1.2.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length469
Molecular weight52218

	---------+----------+----------+----------+----------+
MPYTLSDAHH KLITSHLVDT DPEVDSIIKD EIERQKHSID LIASENFTST   50
SVFDALGTPL SNKYSEGYPG ARYYGGNEHI DRMEILCQQR ALKAFHVTPD   100
KWGVNVQTLS GSPANLQVYQ AIMKPHERLM GLYLPDGGHL SHGYATENRK   150
ISAVSTYFES FPYRVNPETG IIDYDTLEKN AILYRPKVLV AGTSAYCRLI   200
DYKRMREIAD KCGAYLMVDM AHISGLIAAG VIPSPFEYAD IVTTTTHKSL   250
RGPRGAMIFF RRGVRSINPK TGKEVLYDLE NPINFSVFPG HQGGPHNHTI   300
AALATALKQA ATPEFKEYQT QVLKNAKALE SEFKNLGYRL VSNGTDSHMV   350
LVSLREKGVD GARVEYICEK INIALNKNSI PGDKSALVPG GVRIGAPAMT   400
TRGMGEEDFH RIVQYINKAV EFAQQVQQSL PKDACRLKDF KAKVDEGSDV   450
LNTWKKEIYD WAGEYPLAV                                     469