SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSHE1
Systematic nameYBL031W
DescriptionMitotic spindle-associated protein SHE1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38200
SGD YBL031W
Gene Ontology (GO)
Cellular componentGO:0015630microtubule cytoskeletonIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
242Phosphoserine.

Sequence Information
Sequence length338
Molecular weight38015

	---------+----------+----------+----------+----------+
MNDKLQEEHN EKDTTSQING FTPPHMSIDF HSNNNSNIIE TIGVSKRLGN   50
SVLSELDSRA SSKFEFLKDQ SEQQYNGDKN NEPKSGSYNI NEFFQAKHDS   100
QFGQMESLDT HYTLLHTPKR KSQHAIPQDR SDSMKRSRPS RSIPYTTPVV   150
NDITRRIRRL KLRNSLVNGN DIVARARSMQ ANSNINSIKN TPLSKPKPFM   200
HKPNFLMPTT NSLNKINSAH RNTSSSSTAS SIPRSKVHRS ISIRDLHAKT   250
KPVERTPVAQ GTNSQLKNSV SVFDRLYKQT TFSRSTSMNN LSSGTSAKSK   300
EHTNVKTRLV KSKTSGSLSS NLKQSTATGT KSDRPIWR                338