SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSGV1
Systematic nameYPR161C
AliasBUR1
DescriptionSerine/threonine-protein kinase BUR1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP23293
SGD YPR161C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIPI:SGD.
Molecular functionGO:0004693cyclin-dependent protein kinase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
Biological processGO:0006350transcriptionIDA:SGD.
EC number 2.7.11.22,2.7.11.23

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
240Phosphothreonine, by CAK.
241Phosphoserine.
400Phosphoserine.
405Phosphothreonine.
417Phosphoserine.

Sequence Information
Sequence length657
Molecular weight74239

	---------+----------+----------+----------+----------+
MSDNGSPAVL PKTEFNKYKI GKVKSTPAIQ RDAKTNLTYI KLRKRSSEKV   50
YGCTVFQNHY REDEKLGQGT FGEVYKGIHL ETQRQVAMKK IIVSVEKDLF   100
PITAQREITI LKRLNHKNII KLIEMVYDHS PDITNAASSN LHKSFYMILP   150
YMVADLSGVL HNPRINLEMC DIKNMMLQIL EGLNYIHCAK FMHRDIKTAN   200
ILIDHNGVLK LADFGLARLY YGCPPNLKYP GGAGSGAKYT SVVVTRWYRA   250
PELVLGDKQY TTAVDIWGVG CVFAEFFEKK PILQGKTDID QGHVIFKLLG   300
TPTEEDWAVA RYLPGAELTT TNYKPTLRER FGKYLSETGL DFLGQLLALD   350
PYKRLTAMSA KHHPWFKEDP LPSEKITLPT EESHEADIKR YKEEMHQSLS   400
QRVPTAPRGH IVEKGESPVV KNLGAIPRGP KKDDASFLPP SKNVLAKPPP   450
SKIRELHQNP RPYHVNSGYA KTAIPPPAAP AGVNRYGPNN SSRNNRFSGN   500
STAPNNSRNP VNRFHPETNV SSKYNKVPLP LGPQSRYQGN SNESRYKNSP   550
NDSRYHNPRY VNKPETNFNR QPQKYSRQES NAPINKNYNP SNGSRNMAGD   600
HHQGSRPSHP QFPISPSQGQ HQLTSKPIEK KNGSFKDERA KPDESKEFQN   650
SDIADLY                                                  657