SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSGT1
Systematic nameYOR057W
DescriptionProtein SGT1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2   HECTNARSP5   
3        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ08446
SGD YOR057W
Gene Ontology (GO)
Cellular componentGO:0000151ubiquitin ligase complexIDA:SGD.
Biological processGO:0019933cAMP-mediated signalingIGI:SGD.
Biological processGO:0051382kinetochore assemblyIDA:SGD.
Biological processGO:0016567protein ubiquitinationTAS:SGD.
Biological processGO:0000074regulation of progression through cell cycleTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
165Phosphoserine.
168Phosphoserine.
171Phosphoserine.

Sequence Information
Sequence length395
Molecular weight44860

	---------+----------+----------+----------+----------+
MPVEKDLKTA YKALYDEKEP LKALHLYDEI LKGSPTNLTA LIFKAACLEK   50
LYFGFSDWHS DATMENAKEL LDKALMTAEG RGDRSKIGLV NFRYFVHFFN   100
IKDYELAQSY FKKAKNLGYV DDTLPLWEDR LETKLNKKNK KQKDSTNKHT   150
IKPVESIENR GDNNSSHSPI SPLKIETAPQ ESPKFKIDWY QSSTSVTISL   200
FTVNLPESKE QVNIYISPND RRTLSISYQV PKSGSEFQYN AKLSHEVDPK   250
AVSLKIFPKK LEITLSKIDS TQWKKLEEDI LTESSRLSDE GKNSDSATRL   300
LSAETASKER LSYPSSSKKK IDWSKLDIDE EADEEAGSAD SFFQKLYAGA   350
DPDTKRAMMK SFIESNGTAL STDWEDVSKG TVKTSPPEGM EPKHW        395