SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSGS1
Systematic nameYMR190C
AliasTPS1
DescriptionATP-dependent helicase SGS1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP35187
SGD YMR190C
Gene Ontology (GO)
Cellular componentGO:0005730nucleolusIDA:SGD.
Cellular componentGO:0031422RecQ helicase-Topo III complexIDA:SGD.
Molecular functionGO:0004003ATP-dependent DNA helicase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006268DNA unwinding during replicationIDA:SGD.
Biological processGO:0045132meiotic chromosome segregationIMP:SGD.
Biological processGO:0000070mitotic sister chromatid segregationIMP:SGD.
Biological processGO:0001302replicative cell agingIMP:SGD.
Biological processGO:0006974response to DNA damage stimulusIMP:SGD.
Biological processGO:0000722telomere maintenance via recombinationIMP:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1447
Molecular weight163837

	---------+----------+----------+----------+----------+
MVTKPSHNLR REHKWLKETA TLQEDKDFVF QAIQKHIANK RPKTNSPPTT   50
PSKDECGPGT TNFITSIPAS GPTNTATKQH EVMQTLSNDT EWLSYTATSN   100
QYADVPMVDI PASTSVVSNP RTPNGSKTHN FNTFRPHMAS SLVENDSSRN   150
LGSRNNNKSV IDNSSIGKQL ENDIKLEVIR LQGSLIMALK EQSKLLLQKC   200
SIIESTSLSE DAKRLQLSRD IRPQLSNMSI RIDSLEKEII KAKKDGMSKD   250
QSKGRSQVSS QDDNIISSIL PSPLEYNTSS RNSNLTSTTA TTVTKALAIT   300
GAKQNITNNT GKNSNNDSNN DDLIQVLDDE DDIDCDPPVI LKEGAPHSPA   350
FPHLHMTSEE QDELTRRRNM RSREPVNYRI PDRDDPFDYV MGKSLRDDYP   400
DVEREEDELT MEAEDDAHSS YMTTRDEEKE ENELLNQSDF DFVVNDDLDP   450
TQDTDYHDNM DVSANIQESS QEGDTRSTIT LSQNKNVQVI LSSPTAQSVP   500
SNGQNQIGVE HIDLLEDDLE KDAILDDSMS FSFGRQHMPM SHSDLELIDS   550
EKENEDFEED NNNNGIEYLS DSDLERFDEE RENRTQVADI QELDNDLKII   600
TERKLTGDNE HPPPSWSPKI KREKSSVSQK DEEDDFDDDF SLSDIVSKSN   650
LSSKTNGPTY PWSDEVLYRL HEVFKLPGFR PNQLEAVNAT LQGKDVFVLM   700
PTGGGKSLCY QLPAVVKSGK THGTTIVISP LISLMQDQVE HLLNKNIKAS   750
MFSSRGTAEQ RRQTFNLFIN GLLDLVYISP EMISASEQCK RAISRLYADG   800
KLARIVVDEA HCVSNWGHDF RPDYKELKFF KREYPDIPMI ALTATASEQV   850
RMDIIHNLEL KEPVFLKQSF NRTNLYYEVN KKTKNTIFEI CDAVKSRFKN   900
QTGIIYCHSK KSCEQTSAQM QRNGIKCAYY HAGMEPDERL SVQKAWQADE   950
IQVICATVAF GMGIDKPDVR FVYHFTVPRT LEGYYQETGR AGRDGNYSYC   1000
ITYFSFRDIR TMQTMIQKDK NLDRENKEKH LNKLQQVMAY CDNVTDCRRK   1050
LVLSYFNEDF DSKLCHKNCD NCRNSANVIN EERDVTEPAK KIVKLVESIQ   1100
NERVTIIYCQ DVFKGSRSSK IVQANHDTLE EHGIGKSMQK SEIERIFFHL   1150
ITIRVLQEYS IMNNSGFASS YVKVGPNAKK LLTGKMEIKM QFTISAPNSR   1200
PSTSSSFQAN EDNIPVIAQK STTIGGNVAA NPPRFISAKE HLRSYTYGGS   1250
TMGSSHPITL KNTSDLRSTQ ELNNLRMTYE RLRELSLNLG NRMVPPVGNF   1300
MPDSILKKMA AILPMNDSAF ATLGTVEDKY RRRFKYFKAT IADLSKKRSS   1350
EDHEKYDTIL NDEFVNRAAA SSNGIAQSTG TKSKFFGANL NEAKENEQII   1400
NQIRQSQLPK NTTSSKSGTR SISKSSKKSA NGRRGFRNYR GHYRGRK      1447