SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSFA1
Systematic nameYDL168W
AliasSFA
DescriptionS-(hydroxymethyl)glutathione dehydrogenase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP32771
SGD YDL168W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004022alcohol dehydrogenase activityIMP:SGD.
Molecular functionGO:0004327formaldehyde dehydrogenase (glutathione) ac...IDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0046294formaldehyde catabolic processIDA:SGD.
EC number 1.1.1.284,1.1.1.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length386
Molecular weight41042

	---------+----------+----------+----------+----------+
MSAATVGKPI KCIAAVAYDA KKPLSVEEIT VDAPKAHEVR IKIEYTAVCH   50
TDAYTLSGSD PEGLFPCVLG HEGAGIVESV GDDVITVKPG DHVIALYTAE   100
CGKCKFCTSG KTNLCGAVRA TQGKGVMPDG TTRFHNAKGE DIYHFMGCST   150
FSEYTVVADV SVVAIDPKAP LDAACLLGCG VTTGFGAALK TANVQKGDTV   200
AVFGCGTVGL SVIQGAKLRG ASKIIAIDIN NKKKQYCSQF GATDFVNPKE   250
DLAKDQTIVE KLIEMTDGGL DFTFDCTGNT KIMRDALEAC HKGWGQSIII   300
GVAAAGEEIS TRPFQLVTGR VWKGSAFGGI KGRSEMGGLI KDYQKGALKV   350
EEFITHRRPF KEINQAFEDL HNGDCLRTVL KSDEIK                  386