SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSEL1
Systematic nameYML013W
AliasUBX2
DescriptionUBX domain-containing protein 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ04228
SGD YML013W
Gene Ontology (GO)
Cellular componentGO:0030176integral to endoplasmic reticulum membraneIDA:SGD.
Cellular componentGO:0005741mitochondrial outer membraneIDA:SGD.
Molecular functionGO:0030674protein binding, bridgingIPI:SGD.
Biological processGO:0030433ER-associated protein catabolic processIMP:SGD.
Biological processGO:0009306protein secretionIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length584
Molecular weight66761

	---------+----------+----------+----------+----------+
MPVVNHEDSE FHLSHTEEDK LNEFQVITNF PPEDLPDVVR LLRNHGWQLE   50
PALSRYFDGE WKGEPDQMGE PTQTSTPMAE TLVPPALGPR PLLFTASLPV   100
VRPLPANFRN DFRTIGLNGR SNTVWSMFES FSYDGNPFLF ILLLIPRIIN   150
RLSATIFTFF CTLLSLHSIS GGGNSGKPKI SKVPKAPTRE THIPLAEILG   200
DTKDKDAFCE LKSFKPDISF NEALRIAKEE FKFMLLILVG DTYDTDTDTV   250
DVNSKLLLEK ILLNKKTLQY LRKIDNDLII YLKCVHELEP WLVARQLGVR   300
NTPEIFLIAN VANKASHSET LPSQRLSILG KLKVNSLNRF LQSLTNVVEK   350
YTPELVVNKT EMHELRMSRE IKKLQEDAYK KSLEMDRIKA IEKEKSLKHA   400
QDLKLNSTAR QLKWLKACID EIQPFETTGK QATLQFRTSS GKRFVKKFPS   450
MTTLYQIYQS IGCHIYLAVY SSDPAEWSNA LQDKIRQLSA DDDMLCFKEG   500
QLETATATTI EELGHIINNE LTSFDLERGK LEFDFELVSP FPKYTVHPNE   550
HMSVDQVPQL WPNGSLLVEA LDEEDEEDEE NEEQ                    584