SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSEC7
Systematic nameYDR170C
DescriptionProtein transport protein SEC7

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP11075
SGD YDR170C
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005798Golgi-associated vesicleIDA:SGD.
Cellular componentGO:0005770late endosomeIDA:SGD.
Molecular functionGO:0005086ARF guanyl-nucleotide exchange factor activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006888ER to Golgi vesicle-mediated transportIMP:SGD.
Biological processGO:0006891intra-Golgi vesicle-mediated transportIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
212Phosphoserine.
215Phosphoserine.
779Phosphoserine.
807Phosphoserine.
809Phosphoserine.
1220Phosphoserine.
1226Phosphoserine.
1240Phosphothreonine.
1804Phosphoserine.

Sequence Information
Sequence length2009
Molecular weight226886

	---------+----------+----------+----------+----------+
MSEQNSVVNA EKGDGEISSN VETASSVNPS VKPQNAIKEE AKETNGEDQK   50
CKGPENAGST AETKETSNDA TNGMKTPEET EDTNDKRHDD EGEDGDEDED   100
EDEDEDEDNG DEDDEDVDSS SSETSSEDGE DSESVSGEST ESSSGEDEES   150
DESDGNTSNS SSGDESGSEE EEEEEEEEEE EENAGEPAIA HQDSVPTNDS   200
TAPRSTHTRN ISLSSNGSNT NSTIILVKTT LETILNDKDI KKNSNAQKAI   250
ERTLQKFKEF DPQTTNNPHY VDSILVFEAL RASCRTKSSK VQSLALDCLS   300
KLFSFRSLDE TLLVNPPDSL ASNDQRQDAA DGITPPPKQK IIDAAIDTIS   350
DCFQGEGTDD RVELQIVRAL SSCILEEDSS SLCHGASLLK AIRTIYNVFV   400
FSLNPSNQGI AQATLTQIIS SVYDKIDLKQ STSSAVSLST KNHQQQSAIE   450
LSEASENAET PAPLTLENMD KLNDDEERLM DAQQPDSIAI TNQDLAVKDA   500
FLVFRVMAKI CAKPLETELD MRSHAVRSKL LSLHIIYSII KDHIDVFLSH   550
NIFLPGKERV CFIDSIRQYL RLVLSRNAAS PLAPVFEVTL EIMWLLIANL   600
RADFVKEIPV FLTEIYFPIS ELTTSTSQQK RYFLSVIQRI CNDPRTLVEF   650
YLNYDCNPGM PNVMEITVDY LTRLALTRVE ITQTQRSYYD EQISKSLSTY   700
NFSQLPLLTS SNLSSSPDVG QVNLLFPLDF ALKMVSLNCI VSVLRSLSSW   750
AHKALNPNTH TANKVLLNTT SSARQESRSS LSNDVRSSIM TSNDDFKPTY   800
EDEESRSLSS QNIDADDPTQ FENLKLRKTA LSECIAIFNN KPKKAIPVLI   850
KKGFLKDDSP ISIAKWLLET EGLDMAAVGD YLGEGDDKNI AIMHAFVDEF   900
DFTGMSIVDA LRSFLQSFRL PGEGQKIDRF MLKFAERFVD QNPGVFSKAD   950
TAYVLSYSLI MLNTDLHSSQ IKNKMSLQEF LENNEGIDNG RDLPRDFLEG   1000
LFNEIANNEI KLISEQHQAM LSGDTNLVQQ QQSAFNFFNS RDLTREAYNQ   1050
VSKEISSKTE LVFKNLNKNK GGPDVYYAAS HVEHVKSIFE TLWMSFLAAL   1100
TPPFKDYDDI DTTNKCLEGL KISIKIASTF RINDARTSFV GALVQFCNLQ   1150
NLEEIKVKNV NAMVILLEVA LSEGNYLEGS WKDILLVVSQ MERLQLISKG   1200
IDRDTVPDVA QARVANPRVS YESSRSNNTS FFDVWGKKAT PTELAQEKHH   1250
NQTLSPEISK FISSSELVVL MDNIFTKSSE LSGNAIVDFI KALTAVSLEE   1300
IESSENASTP RMFSLQKMVD VCYYNMDRIK LEWTPLWAVM GKAFNKIATN   1350
SNLAVVFFAI DSLRQLSMRF LDIEELSGFE FQHDFLKPFE YTVQNSGNTE   1400
VQEMIIECFR NFILTKSESI KSGWKPILES LQYTARSSTE SIVLKTQLLV   1450
SNDIVTNHFE NVFSQEDAFS ELVGVFREIT KNKRFQKLSL HALESLRKMT   1500
QNVADICFYN ENKTEEERKH NDALLRGKDI FQDVWFPMLF CFNDTIMTAE   1550
DLEVRSRALN YMFDALVAYG GKFNDDFWEK ICKKLLFPIF GVLSKHWEVN   1600
QFNSHDDLSV WLSTTLIQAL RNLIALFTHY FESLNRMLDG FLGLLVSCIC   1650
QENDTIARIG RSCLQQLILQ NVSKFNEYHW NQIGDVFDKL FDLTTANELF   1700
DYDPLQQGRK SSVSHHQTTN DTSQHSDDDS NDRRENDSNI SETVERAHQE   1750
ESSEDVGGDM VETLNGQTKL NNGNSVPTVK DELNPKPASL SIPKKTKHMK   1800
RNESNEDIRR RINIKNSIVV KCVLQLLMIE LLNELFENED FAHCIPYKEA   1850
IRITRLLEKS YEFSRDFNED YGLRTRLVEA RVVDKIPNLL KQETSAAAVL   1900
LDIMFQLYLN DDEKKADLIT RLITICIQVV EGYVSLDDRT MERSINAWRS   1950
VIVEILQGYY EFDDEDFRLY CPAMYALVIQ ILDKSVPTEL RHAIKQFLSR   2000
VGELYLSTD                                                2009