SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSEC63
Systematic nameYOR254C
AliasNPL1, PTL1
DescriptionProtein translocation protein SEC63

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP14906
SGD YOR254C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0031207Sec62/Sec63 complexIPI:SGD.
Molecular functionGO:0008565protein transporter activityIMP:SGD.
Biological processGO:0046967cytosol to ER transportIMP:SGD.
Biological processGO:0006620posttranslational protein targeting to memb...IMP:SGD.
Biological processGO:0006614SRP-dependent cotranslational protein targe...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
512Phosphoserine.

Sequence Information
Sequence length663
Molecular weight75345

	---------+----------+----------+----------+----------+
MPTNYEYDEA SETWPSFILT GLLMVVGPMT LLQIYQIFFG ANAEDGNSGK   50
SKEFNEEVFK NLNEEYTSDE IKQFRRKFDK NSNKKSKIWS RRNIIIIVGW   100
ILVAILLQRI NSNDAIKDAA TKLFDPYEIL GISTSASDRD IKSAYRKLSV   150
KFHPDKLAKG LTPDEKSVME ETYVQITKAY ESLTDELVRQ NYLKYGHPDG   200
PQSTSHGIAL PRFLVDGSAS PLLVVCYVAL LGLILPYFVS RWWARTQSYT   250
KKGIHNVTAS NFVSNLVNYK PSEIVTTDLI LHWLSFAHEF KQFFPDLQPT   300
DFEKLLQDHI NRRDSGKLNN AKFRIVAKCH SLLHGLLDIA CGFRNLDIAL   350
GAINTFKCIV QAVPLTPNCQ ILQLPNVDKE HFITKTGDIH TLGKLFTLED   400
AKIGEVLGIK DQAKLNETLR VASHIPNLKI IKADFLVPGE NQVTPSSTPY   450
ISLKVLVRSA KQPLIPTSLI PEENLTEPQD FESQRDPFAM MSKQPLVPYS   500
FAPFFPTKRR GSWCCLVSSQ KDGKILQTPI IIEKLSYKNL NDDKDFFDKR   550
IKMDLTKHEK FDINDWEIGT IKIPLGQPAP ETVGDFFFRV IVKSTDYFTT   600
DLDITMNMKV RDSPAVEQVE VYSEEDDEYS TDDDETESDD ESDASDYTDI   650
DTDTEAEDDE SPE                                           663