SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSEC61
Systematic nameYLR378C
DescriptionProtein transport protein SEC61

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP32915
SGD YLR378C
Gene Ontology (GO)
Cellular componentGO:0005788endoplasmic reticulum lumenIDA:SGD.
Cellular componentGO:0030176integral to endoplasmic reticulum membraneIDA:SGD.
Cellular componentGO:0031206Sec complex-associated translocon complexTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0008565protein transporter activityTAS:SGD.
Biological processGO:0031204posttranslational protein targeting to memb...TAS:SGD.
Biological processGO:0006616SRP-dependent cotranslational protein targe...TAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length480
Molecular weight52937

	---------+----------+----------+----------+----------+
MSSNRVLDLF KPFESFLPEV IAPERKVPYN QKLIWTGVSL LIFLILGQIP   50
LYGIVSSETS DPLYWLRAML ASNRGTLLEL GVSPIITSSM IFQFLQGTQL   100
LQIRPESKQD RELFQIAQKV CAIILILGQA LVVVMTGNYG APSDLGLPIC   150
LLLIFQLMFA SLIVMLLDEL LSKGYGLGSG ISLFTATNIA EQIFWRAFAP   200
TTVNSGRGKE FEGAVIAFFH LLAVRKDKKR ALVEAFYRTN LPNMFQVLMT   250
VAIFLFVLYL QGFRYELPIR STKVRGQIGI YPIKLFYTSN TPIMLQSALT   300
SNIFLISQIL FQKYPTNPLI RLIGVWGIRP GTQGPQMALS GLAYYIQPLM   350
SLSEALLDPI KTIVYITFVL GSCAVFSKTW IEISGTSPRD IAKQFKDQGM   400
VINGKRETSI YRELKKIIPT AAAFGGATIG ALSVGSDLLG TLGSGASILM   450
ATTTIYGYYE AAAKEGGFTK NLVPGFSDLM                          480