SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSEC31
Systematic nameYDL195W
AliasWEB1
DescriptionProtein transport protein SEC31

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38968
SGD YDL195W
Gene Ontology (GO)
Cellular componentGO:0030127COPII vesicle coatTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0005198structural molecule activityIPI:SGD.
Biological processGO:0006888ER to Golgi vesicle-mediated transportTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
396Phosphothreonine.
836Phosphoserine.
844Phosphoserine.
974Phosphoserine.
975Phosphoserine.
977Phosphoserine.
980Phosphoserine.
992Phosphoserine.
999Phosphoserine.
1050Phosphothreonine.
1053Phosphoserine.

Sequence Information
Sequence length1273
Molecular weight138703

	---------+----------+----------+----------+----------+
MVKLAEFSRT ATFAWSHDKI PLLVSGTVSG TVDANFSTDS SLELWSLLAA   50
DSEKPIASLQ VDSKFNDLDW SHNNKIIAGA LDNGSLELYS TNEANNAINS   100
MARFSNHSSS VKTVKFNAKQ DNVLASGGNN GEIFIWDMNK CTESPSNYTP   150
LTPGQSMSSV DEVISLAWNQ SLAHVFASAG SSNFASIWDL KAKKEVIHLS   200
YTSPNSGIKQ QLSVVEWHPK NSTRVATATG SDNDPSILIW DLRNANTPLQ   250
TLNQGHQKGI LSLDWCHQDE HLLLSSGRDN TVLLWNPESA EQLSQFPARG   300
NWCFKTKFAP EAPDLFACAS FDNKIEVQTL QNLTNTLDEQ ETETKQQESE   350
TDFWNNVSRE ESKEKPSVFH LQAPTWYGEP SPAAHWAFGG KLVQITPDGK   400
GVSITNPKIS GLESNTTLSE ALKTKDFKPL INQRLVKVID DVNEEDWNLL   450
EKLSMDGTEE FLKEALAFDN DESDAQDDAN NEKEDDGEEF FQQIETNFQP   500
EGDFSLSGNI EQTISKNLVS GNIKSAVKNS LENDLLMEAM VIALDSNNER   550
LKESVKNAYF AKYGSKSSLS RILYSISKRE VDDLVENLDV SQWKFISKAI   600
QNLYPNDIAQ RNEMLIKLGD RLKENGHRQD SLTLYLAAGS LDKVASIWLS   650
EFPDLEDKLK KDNKTIYEAH SECLTEFIER FTVFSNFING SSTINNEQLI   700
AKFLEFINLT TSTGNFELAT EFLNSLPSDN EEVKTEKARV LIASGKSLPA   750
QNPATATTSK AKYTNAKTNK NVPVLPTPGM PSTTSIPSMQ APFYGMTPGA   800
SANALPPKPY VPATTTSAPV HTEGKYAPPS QPSMASPFVN KTNSSTRLNS   850
FAPPPNPYAT ATVPATNVST TSIPQNTFAP IQPGMPIMGD YNAQSSSIPS   900
QPPINAVSGQ TPHLNRKAND GWNDLPLKVK EKPSRAKAVS VAPPNILSTP   950
TPLNGIPANA ASTMPPPPLS RAPSSVSMVS PPPLHKNSRV PSLVATSESP   1000
RASISNPYAP PQSSQQFPIG TISTANQTSN TAQVASSNPY APPPQQRVAT   1050
PLSGGVPPAP LPKASNPYAP TATTQPNGSS YPPTGPYTNN HTMTSPPPVF   1100
NKPPTGPPPI SMKKRSNKLA SIEQNPSQGA TYPPTLSSSA SPLQPSQPPT   1150
LASQVNTSAE NVSHEIPADQ QPIVDFLKEE LARVTPLTPK EYSKQLKDCD   1200
KRLKILFYHL EKQDLLTQPT IDCLHDLVAL MKEKKYKEAM VIHANIATNH   1250
AQEGGNWLTG VKRLIGIAEA TLN                                1273