SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSEC28
Systematic nameYIL076W
DescriptionCoatomer subunit epsilon

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP40509
SGD YIL076W
Gene Ontology (GO)
Cellular componentGO:0030126COPI vesicle coatIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006888ER to Golgi vesicle-mediated transportIMP:SGD.
Biological processGO:0006901vesicle coatingIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length296
Molecular weight33829

	---------+----------+----------+----------+----------+
MDYFNIKQNY YTGNFVQCLQ EIEKFSKVTD NTLLFYKAKT LLALGQYQSQ   50
DPTSKLGKVL DLYVQFLDTK NIEELENLLK DKQNSPYELY LLATAQAILG   100
DLDKSLETCV EGIDNDEAEG TTELLLLAIE VALLNNNVST ASTIFDNYTN   150
AIEDTVSGDN EMILNLAESY IKFATNKETA TSNFYYYEEL SQTFPTWKTQ   200
LGLLNLHLQQ RNIAEAQGIV ELLLSDYYSV EQKENAVLYK PTFLANQITL   250
ALMQGLDTED LTNQLVKLDH EHAFIKHHQE IDAKFDELVR KYDTSN       296