SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSEC14
Systematic nameYMR079W
AliasPIT1
DescriptionSEC14 cytosolic factor

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP24280
SGD YMR079W
Gene Ontology (GO)
Cellular componentGO:0005829cytosolIDA:SGD.
Cellular componentGO:0005794Golgi apparatusIDA:SGD.
Molecular functionGO:0008526phosphatidylinositol transporter activityIDA:SGD.
Biological processGO:0006893Golgi to plasma membrane transportIMP:SGD.
Biological processGO:0046470phosphatidylcholine metabolic processIDA:SGD.
Biological processGO:0046488phosphatidylinositol metabolic processTAS:SGD.
Biological processGO:0015914phospholipid transportIDA:SGD.
Biological processGO:0030437sporulation (sensu Fungi)IGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length304
Molecular weight34901

	---------+----------+----------+----------+----------+
MVTQQEKEFL ESYPQNCPPD ALPGTPGNLD SAQEKALAEL RKLLEDAGFI   50
ERLDDSTLLR FLRARKFDVQ LAKEMFENCE KWRKDYGTDT ILQDFHYDEK   100
PLIAKFYPQY YHKTDKDGRP VYFEELGAVN LHEMNKVTSE ERMLKNLVWE   150
YESVVQYRLP ACSRAAGHLV ETSCTIMDLK GISISSAYSV MSYVREASYI   200
SQNYYPERMG KFYIINAPFG FSTAFRLFKP FLDPVTVSKI FILGSSYQKE   250
LLKQIPAENL PVKFGGKSEV DESKGGLYLS DIGPWRDPKY IGPEGEAPEA   300
FSMK                                                     304