SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameSAN1
Systematic nameYDR143C
DescriptionProtein SAN1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP22470
SGD YDR143C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0006325establishment and/or maintenance of chromat...IGI:SGD.
Biological processGO:0006950response to stressIMP:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length610
Molecular weight65618

	---------+----------+----------+----------+----------+
MSESGQEQNR GTNTSPNNAE NNNNSNAASG PLNGGAEQTR NITVSIQYSY   50
FTPERLAHLS NISNNDNNEN NSAASGSTIA NGTGPSFGIG NGGHQPDGAL   100
VLSFRDVPAS TPQDRLNSFI SVAAQLAMER FNRLLNRPKG ISKDEFDKLP   150
VLQVSDLPKA EGPLCSICYD EYEDEVDSTK AKRKRDSENE EESEGTKKRK   200
DNEGAPLRTT ADNDSNPSIT NATVVEPPSI PLTEQQRTLN DEETNPSYKH   250
SPIKLPCGHI FGRECIYKWS RLENSCPLCR QKISESVGVQ RAAQQDTDEV   300
AANEAAFERI RRVLYDPTAV NSTNENSSAP SENTSNTTVP TIGNASSGEQ   350
MLSRTGFFLV PQNGQPLHNP VRLPPNDSDR NGVNGPSSTT QNPPSNSGGS   400
NNNQSPRWVP IPLTLFQFHS PNPNPSASDS SASPSAANGP NSNNTSSDAT   450
DPHHNRLRAV LDHIFNVAQR GTSDTSATTA PGAQTVHNQG RNDSSSSDTT   500
QGSSFLENIS RLTGHFTNGS RDNNNDNNHS NDQQRGGSTG ENNRNNLFSS   550
GVASYRNQNG DVTTVELRNN NSAAFPPTDE NPSQGQGSSS SDTTIHNDVP   600
NDNNEQRSSQ                                                610