SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSAM35
Systematic nameYHR083W
AliasOMP85, TOB38, TOM38
DescriptionSorting assembly machinery 35 kDa subunit

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP14693
SGD YHR083W
Gene Ontology (GO)
Cellular componentGO:0019898extrinsic to membraneIDA:SGD.
Cellular componentGO:0001401mitochondrial sorting and assembly machiner...IDA:SGD.
Molecular functionGO:0005515protein bindingIDA:SGD.
Biological processGO:0045040protein import into mitochondrial outer mem...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length329
Molecular weight37404

	---------+----------+----------+----------+----------+
MVSSFSVPMP VKRIFDTFPL QTYAAQTDKD EAVALEIQRR SYTFTERGGG   50
SSELTVEGTY KLGVYNVFLE ANTGAALATD PWCLFVQLAL CQKNGLVLPT   100
HSQEQTPSHT CNHEMLVLSR LSNPDEALPI LVEGYKKRII RSTVAISEIM   150
RSRILDDAEQ LMYYTLLDTV LYDCWITQII FCASDAQFME LYSCQKLSGS   200
IVTPLDVENS LLQKLSAKSL KISLTKRNKF QFRHREIVKS MQGVYHNHHN   250
SVNQEQVLNV LFENSKQVLL GLKDMLKSDG QPTYLHLKIA SYILCITNVK   300
EPIKLKTFVE NECKELVQFA QDTLKNFVQ                          329