SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSAM1
Systematic nameYLR180W
AliasETH10
DescriptionS-adenosylmethionine synthetase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3        Proteasomal Degradation
4K84;K210        
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP10659
SGD YLR180W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0004478methionine adenosyltransferase activityIGI:SGD.
Biological processGO:0006555methionine metabolic processTAS:SGD.
Biological processGO:0006556S-adenosylmethionine biosynthetic processIMP:SGD.
EC number 2.5.1.6

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length382
Molecular weight41818

	---------+----------+----------+----------+----------+
MAGTFLFTSE SVGEGHPDKI CDQVSDAILD ACLAEDPHSK VACETAAKTG   50
MIMVFGEITT KAQLDYQKIV RDTIKKIGYD DSAKGFDYKT CNVLVAIEQQ   100
SPDIAQGVHE EKDLEDIGAG DQGIMFGYAT DETPEGLPLT ILLAHKLNMA   150
MADARRDGSL AWLRPDTKTQ VTVEYKDDHG RWVPQRIDTV VVSAQHADEI   200
TTEDLRAQLK SEIIEKVIPR DMLDENTKYF IQPSGRFVIG GPQGDAGLTG   250
RKIIVDAYGG ASSVGGGAFS GKDYSKVDRS AAYAARWVAK SLVAAGLCKR   300
VQVQFSYAIG IAEPLSLHVD TYGTATKSDE EIIDIISKNF DLRPGVLVKE   350
LDLARPIYLP TASYGHFTNQ EYPWEKPKTL KF                      382

	* Ubiquitinated lysine is denoted as red color.