SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameSAH1
Systematic nameYER043C
DescriptionAdenosylhomocysteinase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP39954
SGD YER043C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006555methionine metabolic processNAS:SGD.
Biological processGO:0016259selenocysteine metabolic processNAS:SGD.
EC number 3.3.1.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
393Phosphothreonine.

Sequence Information
Sequence length449
Molecular weight49126

	---------+----------+----------+----------+----------+
MSAPAQNYKI ADISLAAFGR KEIELAEHEM PGLMAIRKAY GDVQPLKGAR   50
IAGCLHMTIQ TAVLIETLVA LGAEVTWSSC NIYSTQDHAA AAIAASGVPV   100
FAWKGETEEE YLWCIEQQLF AFKDNKKLNL ILDDGGDLTT LVHEKHPEML   150
EDCFGLSEET TTGVHHLYRM VKEGKLKVPA INVNDSVTKS KFDNLYGCRE   200
SLVDGIKRAT DVMLAGKVAV VAGYGDVGKG CAAALRGMGA RVLVTEIDPI   250
NALQAAMEGY QVVTMEDASH IGQVFVTTTG CRDIINGEHF INMPEDAIVC   300
NIGHFDIEID VAWLKANAKE CINIKPQVDR YLLSSGRHVI LLANGRLVNL   350
GCATGHSSFV MSCSFSNQVL AQIALFKSND KSFREKHIEF QKTGPFEVGV   400
HVLPKILDEA VAKFHLGNLG VRLTKLSKVQ SEYLGIPEEG PFKADHYRY    449