SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRVS167
Systematic nameYDR388W
DescriptionReduced viability upon starvation protein 167

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K481        
2K481MONO HECTNARSP5   
3         
4   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"The Rsp5 ubiquitin ligase binds to and ubiquitinates members of the yeast CIN85-endophilin complex, Sla1-Rvs167."
Stamenova SD, Dunn R, Adler AS, Hicke L.
J Biol Chem. 279(16):16017-25 (2004) [14761940]
[3]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[4]EvidenceUb-conjugate Confirmation
Methodprotein microarray (in vitro)
Reference"Ubiquitination screen using protein microarrays for comprehensive identification of Rsp5 substrates in yeast."
Gupta R, Kus B, Fladd C, Wasmuth J, Tonikian R, Sidhu S, Krogan NJ, Parkinson J, Rotin D.
Mol Syst Biol. 3:116 (2007) [17551511]

Cross References
Swiss-Prot accessionP39743
SGD YDR388W
Gene Ontology (GO)
Cellular componentGO:0030479actin cortical patchTAS:SGD.
Molecular functionGO:0008092cytoskeletal protein bindingIPI:SGD.
Biological processGO:0007121bipolar cellular bud site selectionIMP:SGD.
Biological processGO:0006897endocytosisIMP:SGD.
Biological processGO:0006970response to osmotic stressIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
299Phosphoserine, by FUS3 and PHO85.
321Phosphoserine, by FUS3 and PHO85.
379Phosphoserine, by FUS3 and PHO85.

Sequence Information
Sequence length482
Molecular weight52774

	---------+----------+----------+----------+----------+
MSFKGFTKAV SRAPQSFRQK FKMGEQTEDP VYEDAERRFQ ELEQETKKLS   50
EESKRYSTAV NGMLTHQIGF AKSMEEIFKP ISGKMSDPNA TIPEDNPQGI   100
EASEQYRAIV AELQETLKPD LALVEEKIVT PCQELLKIIT YIRKMATKRN   150
HKKLDLDRHL NTYNKHEKKK EPTAKDEERL YKAQAQVEVA QQEYDYYNDL   200
LKTQLPILFS LEAEFVKPLF VSFYFMQLNI FYTLYNRLQD MKIPYFDLNS   250
DIVESYIAKK GNVEEQTDAL TITHFKLGYS KAKLEMTRRK YGVATAEGSP   300
VSGASSGVGY GAGYDPATAT SPTPTGYGYG AAAPSYAAQP AAQYGTAAAV   350
GTAAAVGTAA GAAAGAVPGT YPQYAAAQSP PLTGLGFQQS PQQQQGPPPA   400
YSNPLTSPVA GTPAAAVAAA PGVETVTALY DYQAQAAGDL SFPAGAVIEI   450
VQRTPDVNEW WTGRYNGQQG VFPGNYVQLN KN                      482

	* Ubiquitinated lysine is denoted as red color.