SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRVB2
Systematic nameYPL235W
AliasTIH2, TIP49B
DescriptionRuvB-like protein 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ12464
SGD YPL235W
Gene Ontology (GO)
Cellular componentGO:0031011INO80 complexIPI:SGD.
Cellular componentGO:0000812SWR1 complexIDA:SGD.
Molecular functionGO:0016887ATPase activityIMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:000636535S primary transcript processingIMP:SGD.
Biological processGO:0006338chromatin remodelingIDA:SGD.
Biological processGO:0006357regulation of transcription from RNA polyme...IMP:SGD.
Biological processGO:0016074snoRNA metabolic processIMP:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length471
Molecular weight51612

	---------+----------+----------+----------+----------+
MSIQTSDPNE TSDLKSLSLI AAHSHITGLG LDENLQPRPT SEGMVGQLQA   50
RRAAGVILKM VQNGTIAGRA VLVAGPPSTG KTALAMGVSQ SLGKDVPFTA   100
IAGSEIFSLE LSKTEALTQA FRKSIGIKIK EETELIEGEV VEIQIDRSIT   150
GGHKQGKLTI KTTDMETIYE LGNKMIDGLT KEKVLAGDVI SIDKASGKIT   200
KLGRSFARSR DYDAMGADTR FVQCPEGELQ KRKTVVHTVS LHEIDVINSR   250
TQGFLALFTG DTGEIRSEVR DQINTKVAEW KEEGKAEIVP GVLFIDEVHM   300
LDIECFSFIN RALEDEFAPI VMMATNRGVS KTRGTNYKSP HGLPLDLLDR   350
SIIITTKSYN EQEIKTILSI RAQEEEVELS SDALDLLTKT GVETSLRYSS   400
NLISVAQQIA MKRKNNTVEV EDVKRAYLLF LDSARSVKYV QENESQYIDD   450
QGNVQISIAK SADPDAMDTT E                                  471