SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRSN1
Systematic nameYMR266W
DescriptionUncharacterized protein RSN1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ03516
SGD YMR266W
Gene Ontology (GO)
Cellular componentGO:0016020membraneIDA:SGD.
Biological processGO:0006893Golgi to plasma membrane transportIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
949Phosphoserine.

Sequence Information
Sequence length953
Molecular weight107673

	---------+----------+----------+----------+----------+
MNSTNSTNST TTATSTNTST QQVVTSLVSN GTIFGVFVIA FLILRIKLKR   50
IYEPKSSFNL INEEKKPEPL PQGVWQWLKP LLKKSDNFVI QQAGLDGYFF   100
LRYLFIIAIY CAVSMSYIFP ILLSINASNG NHESGLNQLA YQNVKHRGRY   150
FAHVFCGWIF FWGFLYIIYR ELYFYTSMKQ AVLASPRYAK KLSSRTVLFQ   200
TVPKQYLSEE EFSKLFDGVK RVWIARGSGS IEAMVKARDN MAIQLEGAET   250
KYLKAALKKI KKLNKKSPQL SVSDNIAEYV PDKKRPHHKI NKVAKFFFGK   300
KVDTISYIKE ELPKLNQKVK ALQEDHENSS PFNSVFVEFE SQYQAQVAAQ   350
ITTYHAPLFM TPVYIGIEPS DVVWFNLRMF WWERLGREVS AVSAIVALVI   400
LWAFPVAFVG MISNITSLTN EVKWLKFIYK LPKQLLGLLT SLAPTVALAV   450
LMSFLPKFIR GMAITQGAPS KQNVEYFTQQ AYFAFQVIQV FLVTTLSSAA   500
TSTVTEIVKE PTKAMDLLAS NLPKASNFFM SYVILQGLSI SSGALLQIVP   550
LILFYVLGAF LDGTVRKKWN RFCGLSSMQW GTAFPVYTNL AVITFSYSII   600
SPLILLFAAV AFFLLYIAYL YNLTYVYQES PDARGIYYPR ALFQTIVGIY   650
IGQICLLGLF AVGKGWGPIV LQVIGICVTV LIHLHLSAAF DHLSKVIPVD   700
TMKPLDGVSD TPSFKNIYKG IESTKVKKNT FGANIDMDGI KELPEFPIKK   750
YHKRSESVTE QQVENSIFSE NTFEYQFNPA NEANADGHAI NAENLIEDVP   800
LLADGDTMKI PPAPWWKRFL KPHIYYSYKA VKSRLPEIYG LVDPDERVND   850
FDISHAYDYP AVSAQCPELW IPRDPFGFSK LLISDVSGVV EMNDENATID   900
ENLKFTLRDV PPPYNDVKDE ANGEANGEFD TASKENNPFA DPKYKEEESR   950
SAV                                                      953