SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPT6
Systematic nameYGL048C
AliasCIM3, CRL3, SUG1, TBPY, TBY1
Description26S protease regulatory subunit 8 homolog

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ01939
SGD YGL048C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0008540proteasome regulatory particle, base subcom...IDA:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
Molecular functionGO:0004175endopeptidase activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylthreonine.

Sequence Information
Sequence length405
Molecular weight45272

	---------+----------+----------+----------+----------+
MTAAVTSSNI VLETHESGIK PYFEQKIQET ELKIRSKTEN VRRLEAQRNA   50
LNDKVRFIKD ELRLLQEPGS YVGEVIKIVS DKKVLVKVQP EGKYIVDVAK   100
DINVKDLKAS QRVCLRSDSY MLHKVLENKA DPLVSLMMVE KVPDSTYDMV   150
GGLTKQIKEI KEVIELPVKH PELFESLGIA QPKGVILYGP PGTGKTLLAR   200
AVAHHTDCKF IRVSGAELVQ KYIGEGSRMV RELFVMAREH APSIIFMDEI   250
DSIGSTRVEG SGGGDSEVQR TMLELLNQLD GFETSKNIKI IMATNRLDIL   300
DPALLRPGRI DRKIEFPPPS VAARAEILRI HSRKMNLTRG INLRKVAEKM   350
NGCSGADVKG VCTEAGMYAL RERRIHVTQE DFELAVGKVM NKNQETAISV   400
AKLFK                                                    405