SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPT5
Systematic nameYOR117W
AliasYTA1
Description26S protease regulatory subunit 6A

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP33297
SGD YOR117W
Gene Ontology (GO)
Cellular componentGO:0008540proteasome regulatory particle, base subcom...IDA:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
Molecular functionGO:0004175endopeptidase activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylalanine.
181Phosphoserine.

Sequence Information
Sequence length434
Molecular weight48256

	---------+----------+----------+----------+----------+
MATLEELDAQ TLPGDDELDQ EILNLSTQEL QTRAKLLDNE IRIFRSELQR   50
LSHENNVMLE KIKDNKEKIK NNRQLPYLVA NVVEVMDMNE IEDKENSEST   100
TQGGNVNLDN TAVGKAAVVK TSSRQTVFLP MVGLVDPDKL KPNDLVGVNK   150
DSYLILDTLP SEFDSRVKAM EVDEKPTETY SDVGGLDKQI EELVEAIVLP   200
MKRADKFKDM GIRAPKGALM YGPPGTGKTL LARACAAQTN ATFLKLAAPQ   250
LVQMYIGEGA KLVRDAFALA KEKAPTIIFI DELDAIGTKR FDSEKSGDRE   300
VQRTMLELLN QLDGFSSDDR VKVLAATNRV DVLDPALLRS GRLDRKIEFP   350
LPSEDSRAQI LQIHSRKMTT DDDINWQELA RSTDEFNGAQ LKAVTVEAGM   400
IALRNGQSSV KHEDFVEGIS EVQARKSKSV SFYA                    434