SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPT3
Systematic nameYDR394W
AliasYNT1, YTA2
Description26S protease regulatory subunit 6B homolog

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP33298
SGD YDR394W
Gene Ontology (GO)
Cellular componentGO:0008540proteasome regulatory particle, base subcom...IDA:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
Molecular functionGO:0004175endopeptidase activityTAS:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
1N-acetylmethionine.
8Phosphothreonine.

Sequence Information
Sequence length428
Molecular weight47970

	---------+----------+----------+----------+----------+
MEELGIVTPV EKAVEEKPAV KSYASLLAQL NGTVNNNSAL SNVNSDIYFK   50
LKKLEKEYEL LTLQEDYIKD EQRHLKRELK RAQEEVKRIQ SVPLVIGQFL   100
EPIDQNTGIV SSTTGMSYVV RILSTLDREL LKPSMSVALH RHSNALVDIL   150
PPDSDSSISV MGENEKPDVT YADVGGLDMQ KQEIREAVEL PLVQADLYEQ   200
IGIDPPRGVL LYGPPGTGKT MLVKAVANST KAAFIRVNGS EFVHKYLGEG   250
PRMVRDVFRL ARENAPSIIF IDEVDSIATK RFDAQTGSDR EVQRILIELL   300
TQMDGFDQST NVKVIMATNR ADTLDPALLR PGRLDRKIEF PYLRDRRERR   350
LIFGTIASKM SLAPEADLDS LIIRNDSLSG AVIAAIMQEA GLRAVRKNRY   400
VILQSDLEEA YATQVKTDNT VDKFDFYK                           428