SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPT2
Systematic nameYDL007W
AliasYHS4, YTA5
Description26S protease regulatory subunit 4 homolog

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2        Proteasomal Degradation
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP40327
SGD YDL007W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0008540proteasome regulatory particle, base subcom...IPI:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
Molecular functionGO:0004175endopeptidase activityTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
180Phosphoserine.

Sequence Information
Sequence length437
Molecular weight48828

	---------+----------+----------+----------+----------+
MGQGVSSGQD KKKKKGSNQK PKYEPPVQSK FGRKKRKGGP ATAEKLPNIY   50
PSTRCKLKLL RMERIKDHLL LEEEFVSNSE ILKPFEKKQE EEKKQLEEIR   100
GNPLSIGTLE EIIDDDHAIV TSPTMPDYYV SILSFVDKEL LEPGCSVLLH   150
HKTMSIVGVL QDDADPMVSV MKMDKSPTES YSDIGGLESQ IQEIKESVEL   200
PLTHPELYEE MGIKPPKGVI LYGAPGTGKT LLAKAVANQT SATFLRIVGS   250
ELIQKYLGDG PRLCRQIFKV AGENAPSIVF IDEIDAIGTK RYDSNSGGER   300
EIQRTMLELL NQLDGFDDRG DVKVIMATNK IETLDPALIR PGRIDRKILF   350
ENPDLSTKKK ILGIHTSKMN LSEDVNLETL VTTKDDLSGA DIQAMCTEAG   400
LLALRERRMQ VTAEDFKQAK ERVMKNKVEE NLEGLYL                 437