SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPT1
Systematic nameYKL145W
AliasCIM5, YTA3
Description26S protease regulatory subunit 7 homolog

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2        Proteasomal Degradation
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP33299
SGD YKL145W
Gene Ontology (GO)
Cellular componentGO:0008540proteasome regulatory particle, base subcom...IPI:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
Molecular functionGO:0004175endopeptidase activityTAS:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
164Phosphoserine.

Sequence Information
Sequence length467
Molecular weight51983

	---------+----------+----------+----------+----------+
MPPKEDWEKY KAPLEDDDKK PDDDKIVPLT EGDIQVLKSY GAAPYAAKLK   50
QTENDLKDIE ARIKEKAGVK ESDTGLAPSH LWDIMGDRQR LGEEHPLQVA   100
RCTKIIKGNG ESDETTTDNN NSGNSNSNSN QQSTDADEDD EDAKYVINLK   150
QIAKFVVGLG ERVSPTDIEE GMRVGVDRSK YNIELPLPPR IDPSVTMMTV   200
EEKPDVTYSD VGGCKDQIEK LREVVELPLL SPERFATLGI DPPKGILLYG   250
PPGTGKTLCA RAVANRTDAT FIRVIGSELV QKYVGEGARM VRELFEMART   300
KKACIIFFDE IDAVGGARFD DGAGGDNEVQ RTMLELITQL DGFDPRGNIK   350
VMFATNRPNT LDPALLRPGR IDRKVEFSLP DLEGRANIFR IHSKSMSVER   400
GIRWELISRL CPNSTGAELR SVCTEAGMFA IRARRKVATE KDFLKAVDKV   450
ISGYKKFSST SRYMQYN                                       467