SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPS7B
Systematic nameYNL096C
Description40S ribosomal protein S7-B

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP48164
SGD YNL096C
Gene Ontology (GO)
Cellular componentGO:0005843cytosolic small ribosomal subunit (sensu Eu...TAS:SGD.
Cellular componentGO:0005732small nucleolar ribonucleoprotein complexIPI:SGD.
Molecular functionGO:0003735structural constituent of ribosomeTAS:SGD.
Biological processGO:0006412translationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
31Phosphoserine.
115Phosphoserine.
117Phosphothreonine.

Sequence Information
Sequence length190
Molecular weight21634

	---------+----------+----------+----------+----------+
MSSVQSKILS QAPSELELQV AKTFIDLESS SPELKADLRP LQIKSIREID   50
VTGGKKALVL FVPVPALSAY HKVQTKLTRE LEKKFPDRHV IFLAERRILP   100
KPSRTSRQVQ KRPRSRTLTA VHDKVLEDMV FPTEIVGKRV RYLVGGNKIQ   150
KVLLDSKDVQ QIDYKLESFQ AVYNKLTGKQ IVFEIPSQTN               190