SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPS6B
Systematic nameYBR181C
AliasRPS102, RPS10A
Description40S ribosomal protein S6

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP02365
SGD YBR181C
Gene Ontology (GO)
Cellular componentGO:0005843cytosolic small ribosomal subunit (sensu Eu...IDA:SGD.
Cellular componentGO:0005732small nucleolar ribonucleoprotein complexIPI:SGD.
Molecular functionGO:0003735structural constituent of ribosomeIDA:SGD.
Biological processGO:0006412translationIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
163Phosphothreonine.
180Phosphothreonine.
232Phosphoserine.
233Phosphoserine.

Sequence Information
Sequence length236
Molecular weight26996

	---------+----------+----------+----------+----------+
MKLNISYPVN GSQKTFEIDD EHRIRVFFDK RIGQEVDGEA VGDEFKGYVF   50
KISGGNDKQG FPMKQGVLLP TRIKLLLTKN VSCYRPRRDG ERKRKSVRGA   100
IVGPDLAVLA LVIVKKGEQE LEGLTDTTVP KRLGPKRANN IRKFFGLSKE   150
DDVRDFVIRR EVTKGEKTYT KAPKIQRLVT PQRLQRKRHQ RALKVRNAQA   200
QREAAAEYAQ LLAKRLSERK AEKAEIRKRR ASSLKA                  236