SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPS4B
Systematic nameYHR203C
AliasRPS7A
Description40S ribosomal protein S4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K168        
2         
3 MULTI       
4 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"A proteomics approach to understanding protein ubiquitination."
Peng J, Schwartz D, Elias JE, Thoreen CC, Cheng D, Marsischky G, Roelofs J, Finley D, Gygi SP.
Nat Biotechnol. 21(8):921-6 (2003) [12872131]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[4]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP05753
SGD YHR203C
Gene Ontology (GO)
Cellular componentGO:0005843cytosolic small ribosomal subunit (sensu Eu...IDA:SGD.
Molecular functionGO:0003735structural constituent of ribosomeIDA:SGD.
Biological processGO:0030490processing of 20S pre-rRNAIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
Biological processGO:0006412translationIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
32Phosphoserine.
115Phosphothreonine.
120Phosphoserine.
247Phosphoserine.

Sequence Information
Sequence length261
Molecular weight29410

	---------+----------+----------+----------+----------+
MARGPKKHLK RLAAPHHWLL DKLSGCYAPR PSAGPHKLRE SLPLIVFLRN   50
RLKYALNGRE VKAILMQRHV KVDGKVRTDT TYPAGFMDVI TLDATNENFR   100
LVYDVKGRFA VHRITDEEAS YKLGKVKKVQ LGKKGVPYVV THDGRTIRYP   150
DPNIKVNDTV KIDLASGKIT DFIKFDAGKL VYVTGGRNLG RIGTIVHKER   200
HDGGFDLVHI KDSLDNTFVT RLNNVFVIGE QGKPYISLPK GKGIKLSIAE   250
ERDRRRAQQG L                                             261

	* Ubiquitinated lysine is denoted as red color.