SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPS23B
Systematic nameYPR132W
AliasRPS28B
Description40S ribosomal protein S23

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32827
SGD YPR132W
Gene Ontology (GO)
Cellular componentGO:0005843cytosolic small ribosomal subunit (sensu Eu...TAS:SGD.
Molecular functionGO:0005515protein bindingIPI:UniProtKB.
Molecular functionGO:0003735structural constituent of ribosomeTAS:SGD.
Biological processGO:0006450regulation of translational fidelityIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length145
Molecular weight16038

	---------+----------+----------+----------+----------+
MGKGKPRGLN SARKLRVHRR NNRWAENNYK KRLLGTAFKS SPFGGSSHAK   50
GIVLEKLGIE SKQPNSAIRK CVRVQLIKNG KKVTAFVPND GCLNFVDEND   100
EVLLAGFGRK GKAKGDIPGV RFKVVKVSGV SLLALWKEKK EKPRS        145