SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPS1B
Systematic nameYML063W
AliasPLC2, RPS10B
Description40S ribosomal protein S1-B

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
3 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP23248
SGD YML063W
Gene Ontology (GO)
Cellular componentGO:0005843cytosolic small ribosomal subunit (sensu Eu...TAS:SGD.
Molecular functionGO:0003735structural constituent of ribosomeTAS:SGD.
Biological processGO:0006412translationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylalanine, partial (By similarity).
76Phosphoserine.
80Phosphoserine.
154Phosphoserine.
159Phosphoserine.
245Phosphoserine.
254Phosphothreonine.

Sequence Information
Sequence length255
Molecular weight28812

	---------+----------+----------+----------+----------+
MAVGKNKRLS RGKKGLKKKV VDPFTRKEWF DIKAPSTFEN RNVGKTLVNK   50
STGLKNASDA LKGRVVEVCL ADLQGSEDHS FRKVKLRVDE VQGKNLLTNF   100
HGMDFTTDKL RSMVRKWQTL IEANVTVKTS DDYVLRIFAI AFTRKQANQV   150
KRHSYAQSSH IRAIRKVISE ILTREVQNST LAQLTSKLIP EVINKEIENA   200
TKDIFPLQNI HVRKVKLLKQ PKFDVGALMA LHGEGSGEEK GKKVSGFKDE   250
VLETV                                                    255