SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPN8
Systematic nameYOR261C
Description26S proteasome regulatory subunit RPN8

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ08723
SGD YOR261C
Gene Ontology (GO)
Cellular componentGO:0008541proteasome regulatory particle, lid subcomp...IDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
314Phosphoserine.
317Phosphoserine.
319Phosphoserine.
321Phosphoserine.

Sequence Information
Sequence length338
Molecular weight38313

	---------+----------+----------+----------+----------+
MSLQHEKVTI APLVLLSALD HYERTQTKEN KRCVGVILGD ANSSTIRVTN   50
SFALPFEEDE KNSDVWFLDH NYIENMNEMC KKINAKEKLI GWYHSGPKLR   100
ASDLKINELF KKYTQNNPLL LIVDVKQQGV GLPTDAYVAI EQVKDDGTST   150
EKTFLHLPCT IEAEEAEEIG VEHLLRDVRD QAAGGLSIRL TNQLKSLKGL   200
QSKLKDVVEY LDKVINKELP INHTILGKLQ DVFNLLPNLG TPDDDEIDVE   250
NHDRINISNN LQKALTVKTN DELMVIYISN LVRSIIAFDD LIENKIQNKK   300
IQEQRVKDKQ SKVSDDSESE SGDKEATAPL IQRKNKKN                338