SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPN6
Systematic nameYDL097C
AliasNAS4
Description26S proteasome regulatory subunit RPN6

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ12377
SGD YDL097C
Gene Ontology (GO)
Cellular componentGO:0008541proteasome regulatory particle, lid subcomp...IPI:SGD.
Molecular functionGO:0005198structural molecule activityIMP:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
287Phosphothreonine.

Sequence Information
Sequence length434
Molecular weight49774

	---------+----------+----------+----------+----------+
MSLPGSKLEE ARRLVNEKQY NEAEQVYLSL LDKDSSQSSA AAGASVDDKR   50
RNEQETSILE LGQLYVTMGA KDKLREFIPH STEYMMQFAK SKTVKVLKTL   100
IEKFEQVPDS LDDQIFVCEK SIEFAKREKR VFLKHSLSIK LATLHYQKKQ   150
YKDSLALIND LLREFKKLDD KPSLVDVHLL ESKVYHKLRN LAKSKASLTA   200
ARTAANSIYC PTQTVAELDL MSGILHCEDK DYKTAFSYFF ESFESYHNLT   250
THNSYEKACQ VLKYMLLSKI MLNLIDDVKN ILNAKYTKET YQSRGIDAMK   300
AVAEAYNNRS LLDFNTALKQ YEKELMGDEL TRSHFNALYD TLLESNLCKI   350
IEPFECVEIS HISKIIGLDT QQVEGKLSQM ILDKIFYGVL DQGNGWLYVY   400
ETPNQDATYD SALELVGQLN KVVDQLFEKA SVLY                    434