SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPN4
Systematic nameYDL020C
AliasSON1, UFD5
DescriptionProtein RPN4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2  RAD6RINGSPRFUBR2   
3K123;K132;K137;K141;K158;K187MONO, MULTI(K48-linked)RAD6RINGSPRFUBR2   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Proteasomal degradation of RPN4 via two distinct mechanisms, ubiquitin-dependent and -independent."
Ju D, Xie Y.
J Biol Chem. 279(23):23851-4 (2004) [15090546]
[2]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Rpn4 is a physiological substrate of the Ubr2 ubiquitin ligase."
Wang L, Mao X, Ju D, Xie Y.
J Biol Chem. 279(53):55218-23 (2004) [15504724]
[3]EvidenceHigh MW Product Confirmation
Methodautoradiography
Reference"Identification of the preferential ubiquitination site and ubiquitin-dependent degradation signal of Rpn4."
Ju D, Xie Y.
J Biol Chem. 281(16):10657-62 (2006) [16492666]

Cross References
Swiss-Prot accessionQ03465
SGD YDL020C
Gene Ontology (GO)
Cellular componentGO:0005838proteasome regulatory particle (sensu Eukar...IGI:SGD.
Molecular functionGO:0003700transcription factor activityIDA:SGD.
Molecular functionGO:0016563transcriptional activator activityIDA:SGD.
Biological processGO:0000086G2/M transition of mitotic cell cycleIMP:SGD.
Biological processGO:0045739positive regulation of DNA repairIMP:SGD.
Biological processGO:0045732positive regulation of protein catabolic pr...IMP:SGD.
Biological processGO:0045941positive regulation of transcriptionIDA:SGD.
Biological processGO:0046685response to arsenicIEP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length531
Molecular weight60153

	---------+----------+----------+----------+----------+
MASTELSLKR TLTDILEDEL YHTNPGHSQF TSHYQNYHPN ASITPYKLVN   50
KNKENNTFTW NHSLQHQNES SAASIPPQQT YHFPIFNKYA DPTLTTTTSF   100
TTSEATANDR QINNVHLIPN EIKGASETPL QKTVNLKNIM KVSDPYVPTR   150
NTFNYDVKIS NDFFDNGDNL YGNDEEVLFY EDNYNPKMQW SLQDNSAAIN   200
NEDARAIFNN EFDSDDDDIS DDEEDEIEEN CLQQEQHQEE PLLSLDVTPI   250
SMFGSDQKTG RAKSSSHLFN EYSYVDSNMD SISSVVSEDL LDERGHEKIE   300
DEDEDNDLDE DDIYDISLLK NRRKQSFVLN KNTIDFERFP SPSTSANVPS   350
TATTGKRKPA KSSSNRSCVS NSNENGTLER IKKPTSAVVS SNASRRKLIN   400
YTKKHLSSHS STNSNSKPST ASPSAHTSSS DGNNEIFTCQ IMNLITNEPC   450
GAQFSRSYDL TRHQNTIHAK RKIVFRCSEC IKILGSEGYQ KTFSRLDALT   500
RHIKSKHEDL SLEQRQEVTK FAKANIGYVM G                       531

	* Ubiquitinated lysine is denoted as red color.