SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPN2
Systematic nameYIL075C
AliasSEN3
Description26S proteasome regulatory subunit RPN2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP32565
SGD YIL075C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0008540proteasome regulatory particle, base subcom...IPI:SGD.
Molecular functionGO:0030674protein binding, bridgingIPI:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.

Sequence Information
Sequence length945
Molecular weight104232

	---------+----------+----------+----------+----------+
MSLTTAAPLL ALLRENQDSV KTYALESINN VVDQLWSEIS NELPDIEALY   50
DDDTFSDREM AALIASKVYY NLGEYESAVK YALAAKDRFD IDEKSQFVET   100
IVSKSIEMYV QEASKQYTKD EQFYTKDIID PKLTSIFERM IEKCLKASEL   150
KLALGIALEG YRLDIIESAL KSKLDQDSTS ENVKIINYLL TLAITTVTNS   200
KFRSSILRKS FDFLMNMPNC DYLTLNKVVV NLNDAGLALQ LFKKLKEEND   250
EGLSAQIAFD LVSSASQQLL EILVTELTAQ GYDPALLNIL SGLPTCDYYN   300
TFLLNNKNID IGLLNKSKSS LDGKFSLFHT AVSVANGFMH AGTTDNSFIK   350
ANLPWLGKAQ NWAKFTATAS LGVIHKGNLL EGKKVMAPYL PGSRASSRFI   400
KGGSLYGLGL IYAGFGRDTT DYLKNIIVEN SGTSGDEDVD VLLHGASLGI   450
GLAAMGSANI EVYEALKEVL YNDSATSGEA AALGMGLCML GTGKPEAIHD   500
MFTYSQETQH GNITRGLAVG LALINYGRQE LADDLITKML ASDESLLRYG   550
GAFTIALAYA GTGNNSAVKR LLHVAVSDSN DDVRRAAVIA LGFVLLRDYT   600
TVPRIVQLLS KSHNAHVRCG TAFALGIACA GKGLQSAIDV LDPLTKDPVD   650
FVRQAAMIAL SMILIQQTEK LNPQVADINK NFLSVITNKH QEGLAKFGAC   700
VAQGIMNAGG RNVTIQLENA DTGTLDTKSV VGLVMFSQFW YWFPLAHFLS   750
LSFTPTTVIG IRGSDQAIPK FQMNCYAKED AFSYPRMYEE ASGKEVEKVA   800
TAVLSTTARA KARAKKTKKE KGPNEEEKKK EHEEKEKERE TNKKGIKETK   850
ENDEEFYKNK YSSKPYKVDN MTRILPQQSR YISFIKDDRF VPVRKFKGNN   900
GVVVLRDREP KEPVALIETV RQMKDVNAPL PTPFKVDDNV DFPSA        945