SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPN10
Systematic nameYHR200W
AliasMCB1, SUN1
Description26S proteasome regulatory subunit RPN10

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38886
SGD YHR200W
Gene Ontology (GO)
Cellular componentGO:0008540proteasome regulatory particle, base subcom...IPI:SGD.
Molecular functionGO:0004175endopeptidase activityTAS:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
259Phosphoserine.

Sequence Information
Sequence length268
Molecular weight29747

	---------+----------+----------+----------+----------+
MVLEATVLVI DNSEYSRNGD FPRTRFEAQI DSVEFIFQAK RNSNPENTVG   50
LISGAGANPR VLSTFTAEFG KILAGLHDTQ IEGKLHMATA LQIAQLTLKH   100
RQNKVQHQRI VAFVCSPISD SRDELIRLAK TLKKNNVAVD IINFGEIEQN   150
TELLDEFIAA VNNPQEETSH LLTVTPGPRL LYENIASSPI ILEEGSSGMG   200
AFGGSGGDSD ANGTFMDFGV DPSMDPELAM ALRLSMEEEQ QRQERLRQQQ   250
QQQDQPEQSE QPEQHQDK                                      268