SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameRPN1
Systematic nameYHR027C
AliasHRD2, NAS1, RPD1
Description26S proteasome regulatory subunit RPN1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP38764
SGD YHR027C
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Cellular componentGO:0008540proteasome regulatory particle, base subcom...IPI:SGD.
Molecular functionGO:0030674protein binding, bridgingIPI:SGD.
Biological processGO:0006511ubiquitin-dependent protein catabolic processTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
19Phosphoserine.
24Phosphothreonine.

Sequence Information
Sequence length993
Molecular weight109492

	---------+----------+----------+----------+----------+
MVDESDKKQQ TIDEQSQISP EKQTPNKKDK KKEEEEQLSE EDAKLKTDLE   50
LLVERLKEDD SSLYEASLNA LKESIKNSTS SMTAVPKPLK FLRPTYPDLC   100
SIYDKWTDPN LKSSLADVLS ILAMTYSENG KHDSLRYRLL SDVSDFEGWG   150
HEYIRHLALE IGEVYNDQVE KDAEDETSSD GSKSDGSAAT SGFEFSKEDT   200
LRLCLDIVPY FLKHNGEEDA VDLLLEIESI DKLPQFVDEN TFQRVCQYMV   250
ACVPLLPPPE DVAFLKTAYS IYLSQNELTD AIALAVRLGE EDMIRSVFDA   300
TSDPVMHKQL AYILAAQKTS FEYEGVQDII GNGKLSEHFL YLAKELNLTG   350
PKVPEDIYKS HLDNSKSVFS SAGLDSAQQN LASSFVNGFL NLGYCNDKLI   400
VDNDNWVYKT KGDGMTSAVA SIGSIYQWNL DGLQQLDKYL YVDEPEVKAG   450
ALLGIGISAS GVHDGEVEPA LLLLQDYVTN PDTKISSAAI LGLGIAFAGS   500
KNDEVLGLLL PIAASTDLPI ETAAMASLAL AHVFVGTCNG DITTSIMDNF   550
LERTAIELKT DWVRFLALAL GILYMGQGEQ VDDVLETISA IEHPMTSAIE   600
VLVGSCAYTG TGDVLLIQDL LHRLTPKNVK GEEDADEEET AEGQTNSISD   650
FLGEQVNEPT KNEEAEIEVD EMEVDAEGEE VEVKAEITEK KNGESLEGEE   700
IKSEEKKGKS SDKDATTDGK NDDEEEEKEA GIVDELAYAV LGIALIALGE   750
DIGKEMSLRH FGHLMHYGNE HIRRMVPLAM GIVSVSDPQM KVFDTLTRFS   800
HDADLEVSMN SIFAMGLCGA GTNNARLAQL LRQLASYYSR EQDALFITRL   850
AQGLLHLGKG TMTMDVFNDA HVLNKVTLAS ILTTAVGLVS PSFMLKHHQL   900
FYMLNAGIRP KFILALNDEG EPIKVNVRVG QAVETVGQAG RPKKITGWIT   950
QSTPVLLNHG ERAELETDEY ISYTSHIEGV VILKKNPDYR EEE          993