SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameRPL8A
Systematic nameYHL033C
AliasMAK7, RPL4A
Description60S ribosomal protein L8-A

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP17076
SGD YHL033C
Gene Ontology (GO)
Cellular componentGO:0005842cytosolic large ribosomal subunit (sensu Eu...TAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003735structural constituent of ribosomeTAS:SGD.
Biological processGO:0006412translationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
126Phosphoserine.
216Phosphoserine.

Sequence Information
Sequence length256
Molecular weight28125

	---------+----------+----------+----------+----------+
MAPGKKVAPA PFGAKSTKSN KTRNPLTHST PKNFGIGQAV QPKRNLSRYV   50
KWPEYVRVQR QKKILSIRLK VPPTIAQFQY TLDRNTAAET FKLFNKYRPE   100
TAAEKKERLT KEAAAVAEGK SKQDASPKPY AVKYGLNHVV ALIENKKAKL   150
VLIANDVDPI ELVVFLPALC KKMGVPYAIV KGKARLGTLV NQKTSAVAAL   200
TEVRAEDEAA LAKLVSTIDA NFADKYDEVK KHWGGGILGN KAQAKMDKRA   250
KNSDSA                                                   256