SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameRPL6B
Systematic nameYLR448W
AliasRPL16B, RPL17B, YL16B
Description60S ribosomal protein L6-B

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP05739
SGD YLR448W
Gene Ontology (GO)
Molecular functionGO:0003723RNA bindingIDA:SGD.
Biological processGO:0000027ribosomal large subunit assembly and mainte...IMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
162Phosphoserine.

Sequence Information
Sequence length176
Molecular weight19986

	---------+----------+----------+----------+----------+
MTAQQAPKWY PSEDVAAPKK TRKAVRPQKL RASLVPGTVL ILLAGRFRGK   50
RVVYLKHLED NTLLVTGPFK VNGVPLRRVN ARYVIATSTK VSVEGVNVEK   100
FNVEYFAKEK LTKKEKKEAN LFPEQQTKEI KTERVEDQKV VDKALLAEIK   150
KTPLLKQYLS ASFSLKNGDK PHLLKF                             176