SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameRPL1B
Systematic nameYGL135W
AliasSSM1B, SSM2
Description60S ribosomal protein L1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP53030
SGD YGL135W
Gene Ontology (GO)
Cellular componentGO:0005842cytosolic large ribosomal subunit (sensu Eu...TAS:SGD.
Molecular functionGO:0003735structural constituent of ribosomeTAS:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
Biological processGO:0006412translationTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylserine.
7Phosphoserine.
79Phosphoserine.

Sequence Information
Sequence length217
Molecular weight24486

	---------+----------+----------+----------+----------+
MSKITSSQVR EHVKELLKYS NETKKRNFLE TVELQVGLKN YDPQRDKRFS   50
GSLKLPNCPR PNMSICIFGD AFDVDRAKSC GVDAMSVDDL KKLNKNKKLI   100
KKLSKKYNAF IASEVLIKQV PRLLGPQLSK AGKFPTPVSH NDDLYGKVTD   150
VRSTIKFQLK KVLCLAVAVG NVEMEEDVLV NQILMSVNFF VSLLKKNWQN   200
VGSLVVKSSM GPAFRLY                                       217